TOXICITY ASSESSMENT OF STEM CELLS IN SOFT TISSUE DEFECTS IN EXPERIMENTAL ANIMALS
Abstract
The article presents an experimental analysis of cytoxicity of stem cells application in deep soft tissue defects in experimental animals (rats). The dynamics of animal weight and relative weight of internal organs also did not undergo statistically significant deviations in comparison with control and reference values, which indicates the safety of the used stem cells for the organism of experimental animals.
References
Al-Azab M, Safi M, Idiiatullina E, Al-Shaebi F, Zaky MY. Aging of mesenchymal stem cell: machinery, markers, and strategies of fighting. Cell Mol Biol Lett. 2022 Aug 19;27(1):69. doi: 10.1186/s11658-022-00366-0. PMID: 35986247; PMCID: PMC9388978.
Goldenberg D, McLaughlin C, Koduru SV, Ravnic DJ. Regenerative Engineering: Current Applications and Future Perspectives. Front Surg. 2021 Nov 3; 8:731031. doi: 10.3389/fsurg.2021.731031. eCollection 2021.
Khramova N.V., Xusanova Y. B., Makhmudov A. A. Regarding the regeneration of superficial skin defects. Central Asian Journal of Medicine. Tashkent, 2021; 2: 115-118.
Li M, Yu Y, Xue K, Li J, Son G, Wang J, Qian W, Wang S, Zheng J, Yang C, Ge J. Genistein mitigates senescence of bone marrow mesenchymal stem cells via ERRa-mediated mitochondrial biogenesis and mitophagy in ovariectomized rats. Redox Biol. 2023 May; 61:102649. doi: 10.1016/j.redox.2023.102649. Epub 2023 Feb 27. PMID: 36871183; PMCID: PMC9995482.
Shanti RM, Li WJ, Nesti LJ, Wang X, Tuan RS. Adult mesenchymal stem cells: biological properties, characteristics, and applications in maxillofacial surgery. J Oral Maxillofac Surg. 2007 Aug;65(8):1640-7. doi: 10.1016/j.joms.2007.04.008. PMID: 17656295.
Vatani M, Beigi MH, Ejeian F, Mottaghi A, Yadegari-Naeini A, Nasr-Esfahani MH. Cytotoxicity Evaluation of The Bioresorbable and Titanium Plates/Screws Used in Maxillofacial Surgery on Gingival Fibroblasts and Human Mesenchymal Bone Marrow Stem Cells. Cell J. 2020 Oct;22(3):310-318. doi: 10.22074/cellj.2020.6409. Epub 2019 Dec 15. PMID: 31863656; PMCID: PMC6947002.
Wang Y, Liu Y, Chen E, Pan Z. The role of mitochondrial dysfunction in mesenchymal stem cell senescence. Cell Tissue Res. 2020 Dec;382(3):457-462. doi: 10.1007/s00441-020-03272-z. Epub 2020 Aug 17. PMID: 32803322.
Weng Z, Wang Y, Ouchi T, Liu H, Qiao X, Wu C, Zhao Z, Li L, Li B. Mesenchymal Stem/Stromal Cell Senescence: Hallmarks, Mechanisms, and Combating Strategies. Stem Cells Transl Med. 2022 Apr 29;11(4):356-371. doi: 10.1093/stcltm/szac004. PMID: 35485439; PMCID: PMC9052415.
Zhang Y, Guo L, Han S, Chen L, Li C, Zhang Z, Hong Y, Zhang X, Zhou X, Jiang D, Liang X, Qiu J, Zhang J, Li X, Zhong S, Liao C, Yan B, Tse HF, Lian Q. Adult mesenchymal stem cell ageing interplays with depressed mitochondrial Ndufs6. Cell Death Dis. 2020 Dec 15;11(12):1075. doi: 10.1038/s41419-020-03289-w. PMID: 33323934; PMCID: PMC7738680.
Zhao H, Zhao H, Ji S. A Mesenchymal stem cell Aging Framework, from Mechanisms to Strategies. Stem Cell Rev Rep. 2024 Aug;20(6):1420-1440. doi: 10.1007/s12015-024-10732-4. Epub 2024 May 10. PMID: 38727878.
